Study the Thorax Ossification Centers in Partridge Embryos by Alizarin Red and Alcian Blue Double Staining Techniques
نویسندگان
چکیده
منابع مشابه
Modified double skeletal staining protocols with Alizarin red and Alcian blue in laboratory animals
Abstract Purpose: Skeletal staining is a way to study the effect of many chemical or herbal substances on development of bones and cartilages in order to record the level of probable deformity.This article aimed to present a modified protocol to make all skeletal studies on laboratory animals possible. Materials and Methods: The important notes about skeletal staining were fixing by ethan...
متن کاملmodified double skeletal staining protocols with alizarin red and alcian blue in laboratory animals
abstract purpose: skeletal staining is a way to study the effect of many chemical or herbal substances on development of bones and cartilages in order to record the level of probable deformity.this article aimed to present a modified protocol to make all skeletal studies on laboratory animals possible. materials and methods: the important notes about skeletal staining were fixing by ethanol and...
متن کاملTwo Separated Protocols with the Most Important Comments for Skeletal Staining in Embryonic and Adulthood Period in Laboratory Animals
Introduction: Skeletal staining is an important part of anatomical studies which can evaluate macroscopic disorders in bones and cartilages development. The aim of the present article is to illustrate two distinct protocols by all details in order to stain skeletal system of the lab animals in all ages of their lives so it becomes easy for future studies in this field. Methods: In this pa...
متن کاملTemperature-dependent staining reaction of chromatin by Alcian blue.
After treatment with Alcian blue at high temperatures, nuclei from chicken blood smears showed an intense staining reaction, which proved to be dependent on the DNA content of chromatin. The possibility that interactions between the phthalocyanine chromophore and unpaired bases accounts for this temperature-dependent staining of chromatin is briefly discussed.
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Difficulties in obtaining reproducible and accurate enumeration of circulating basophils with existing techniques have hampered investigation of this infrequent cell population. A new basophil staining method is described that employs alcian blue dye for staining of heparin within basophils at low pH and in the presence of lanthanum ions. Basophil recognition is facilitated by reducing nonspeci...
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ژورنال
عنوان ژورنال: Egyptian Journal of Veterinary Sciences
سال: 2021
ISSN: 2357-089X
DOI: 10.21608/ejvs.2020.38380.1184